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Azusa HSCI 100 – Define polymerase chain reaction (PCR)

Week 7 Assignment1. Define polymerase chain reaction (PCR). Demonstrate one cycle of the PCR process starting withone piece of DNA fragment. In the drawing, label template DNA, primers, dNTPs, and DNApolymerase. 2. Compare and contrast Southern blotting, Northern blotting, and Western blotting. 3. Severe combined immunodeficiency disease (SCID) is fatal at an early age. SCID is caused by thelack of the enzyme adenosine deaminase, which is encoded by the gene ADA. Suppose youwant to use a gene therapy method to treat the disease. You have DNA that contains the ADAgene and a virus that infects human cells. Design a protocol to get the ADA gene into the viralDNA. 4. Define restriction fragment length polymorphism (RFLP) analysis. The information generatedfrom RFLP is useful in criminal investigations. Suppose some DNA samples are taken from acrime scene, suspect, and the victim. The samples are amplified by PCR, and then subjected toRFLP analysis. Below is a gel electrophoresis of the RFLP results. D1 is from defendant 1; D2 isfrom defendant 2; V is from the victim; and S1, S2, and S3 are from the crime scene. Speculateon how these results would have an impact on each defendant. 5. Define genomics and proteomics. Name two techniques that are used in each field of study.Protein is translated from mRNA, which is transcribed from the gene. However, the proteome ismuch larger than the genome for a given organism. Give two reasons why there are moreproteins than type of genes.

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